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Representative AF4 separation of EVPs prepared from B16F10 cells using differential ultracentrifugation
(A) Representative AF4 fractionation profile showing hydrodynamic radius (Rh) and fractograms of UV absorbance and QELS plotted against time.
(B) Representative negative staining TEM images of combined fractions resolved by AF4 for corresponding exomeres, Exo-S, and Exo-L subpopulations. Colored arrows: representative EVPs in each subpopulation (adapted from Zhang et al., 2018).
