Table 2.
Increased EVP yields from dissected tissue explant cultures versus whole tissue cultures
| Tissue | EVP concentration by BCA (ng/μL) |
EVP size by NTA (nm) |
The number of EVP particles by NTA (particles/mL) |
|||
|---|---|---|---|---|---|---|
| Whole | Dissected | Whole | Dissected | Whole | Dissected | |
| Human | ||||||
| Colon | 353.3 | 741.8 | 109 (±3) | 107 (±2) | 1.3 × 108 (±1.0 × 107) | 3.1 × 108 (±2.0 × 107) |
| Mouse | ||||||
| Brain | 29.1 | 329.4 | 134 (±6) | 144 (±4) | 3.2 × 108 (±2.9 × 107) | 4.9×108 (±1.8 × 107) |
| Kidney | 12.0 | 42.2 | 140 (±4) | 159 (±16) | 6.8 × 108 (±7.6×107) | 2.6 × 108 (±1.9×107) |
| Liver | 83.1 | 247.2 | 116 (±5) | 143 (±14) | 2.3 × 108 (±1.0 × 107) | 3.5 × 108 (±2.9 × 107) |
| Lung | 11.1 | 209.8 | 120 (±1) | 115 (±1) | 4.7 × 109 (±5.4 × 107) | 5.6 × 109 (±3.9 × 107) |
| Pancreas | 44.6 | 188.5 | 178 (±27) | 202 (±53) | 7.2 × 107 (±8.1 × 106) | 1.4 × 108 (±1.5 × 107) |
| Spleen | 7.7 | 92.0 | 145 (±14) | 163 (±27) | 6.1 × 108 (±2.9 × 107) | 5.3×108 (±2.0 × 107) |
| Thymus | 37.0 | 145.1 | 135 (±5) | 138 (±11) | 4.4 × 108 (±4.6 × 107) | 2.6 × 108 (±1.2 × 108) |
| White adipose tissue | 0.2 | 1,299.6 | 134 (±8) | 130 (±10) | 9.9 × 107 (±1.2 × 107) | 6.4 × 108 (±6.2 × 107) |
| Brown adipose tissue | 7.1 | 138.6 | 113 (±8) | 153 (±16) | 2.2 × 108 (±3.3 × 107) | 2.0 × 108 (±2.3 × 107) |
EVP size is similar between the two conditions. EVP protein concentration was measured by BCA (ng/μL, left), EVP size (nm, middle) and concentration (number of EVP/μg tissue) were measured by Nanosight (right) for human colon tissue and murine pancreas, white adipose tissue (WAT), brown adipose tissue (BAT), lung, brain, spleen, thymus, kidney, and liver.