| Reagent | Final concentration | Amount |
|---|---|---|
| Sucrose | 30% | 15 g |
| Tris (Trizma base, Sigma) | 20 mM | 1.2 g |
| D2O | n/a | Fill to 48 mL |
| HCl | n/a | 1–2 mL |
| Total | n/a | 50 mL |
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