Figure 3.

Fitting of mRNA temporal profile

Spline interpolation (solid line) for ColIV (A) and Ndg (B) mRNA data, respectively, allowing to infer the intermediate data points between mRNA experimental acquisitions (circles). A poor fitting example is shown in (C): the data point at 12 h and 14 h were deleted to simulate poor fitting quality for the unsmoothed ColIV mRNA profile with the same interpolating spline function as of (A) (dash line, gray square). The data points at later time are not frequent enough to capture the curve plateau at equilibrium. A.U., arbitrary units (relative measure over time).