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Morphology of rat MSCs and quantification of MSC-CM. (A, B) Phase contrast images of rMSCs showed spindle shaped, fibroblast-like morphology. rMSCs cultured for 3 days (A) and for 7days (B). Bar = 200μm. (C, D) Mineral depositions by Alizarin red S staining. rMSCs cultured with DMEM(-) showed no mineral depositions (C), however many depositions were observed in rMSCs cultured with osteogenic medium (D) (x40).
