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. 2021 Feb 16;296:100428. doi: 10.1016/j.jbc.2021.100428

Figure 3.

Figure 3

SIK3 catalytic activity is critical for IL-13, GM-CSF, and TNF secretion in FLMC.A–F, FLMC from WT embryos, embryos expressing the kinase-inactive mutants of SIK3 (SIK3[T163A]) or both SIK2 and SIK3 (SIK2[T175A]/SIK3[T163A]) as well as SIK2/SIK3 double KO embryos were stimulated for the times indicated with 10 ng/ml IL-33. The concentrations of IL-13 (A, D), GM-CSF (B, E), and TNF (C, F) in the cell culture medium were then analyzed. The results in A–F are from a single experiment using FLMC from four WT, four SIK3[T163A], six SIK2[T175A]/SIK3[T163A], and four SIK2/3 KO mice and are plotted as the mean and standard deviation. Similar results were obtained in two independent experiments. The statistical analysis is represented by repeated measures two-way ANOVA with Dunnett’s multiple comparison test comparing all mutants to WT mast cells; ∗ p < 0.05, ∗∗ p < 0.01. BMMC, bone marrow-derived mast cell; FLMC, fetal liver–derived mast cell; GM-CSF, granulocyte-macrophage colony stimulating factor; IL, interleukin; KO, knock-out; SIK, salt-inducible kinase; TNF, tumor necrosis factor.