Fig. 1.

IRF2 was increased in HCC and positive correlated with β-catenin expression. A. The relative RNA expression of IRF2 in 14 HCC samples and 14 paired adjacent normal samples from our hospital were detected by real-time PCR. Student's t-test; B. The overall survival of patients with low IRF2 expression (n = 185) and high IRF2 expression (n = 184) in 369 HCC tissue samples. Student's t-test, Kaplan–Meier method and the log-rank test were used; C. Correlation between the RNA expression of IRF2 and β-catenin in 14 HCC samples. The Pearson correlation coefficient (r²) and P value are shown; D. Immunofluorescence staining of IRF2 (red), β-catenin (green) and DAPI (blue) in HCC tissues and paired adjacent normal tissues; E. Immunoblotting of IRF2 expression and β-catenin expression in 8 HCC samples and 8 paired adjacent normal samples from our hospital. Student's t-test. F. Correlation between the protein expression of IRF2 and β-catenin in 8 HCC samples as described in Fig. 1F.