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. 2021 Mar 15;14(6):101059. doi: 10.1016/j.tranon.2021.101059

Fig. 2.

Fig 2

IRF2 promotes cellular proliferation and inhibits cellular apoptosis in HCC. A. Immunoblotting of Bcl-2, survivin, 89 kD PARP, 116 kD PARP, IRF2 and β-catenin in HepG2 cells infected with negative control and IRF2 overexpression adenoviruses or control and IRF2-targeting siRNA (top panel). Immunoblotting of cleaved Caspase-3, Bcl-2, survivin, IRF2 and β-catenin in Huh7 cells infected with negative control and IRF2 overexpression adenoviruses or control and IRF2-targeting siRNA (bottom panel). β-actin was used as loading control. The quantified levels of these proteins were described below; B. Immunofluorescence staining of TUNEL (green) and DAPI (blue) in HepG2 and Huh7 cells infected with negative control and IRF2 overexpression adenoviruses, or HepG2 and Huh7 cells transfected with control and IRF2-targeting siRNA; C. Cell viability of HepG2 and Huh7 cells infected with negative control and IRF2 overexpression adenoviruses, or HepG2 and Huh7 cells transfected with control and IRF2-targeting siRNA. Student's t-test.