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. 2021 Mar 21;6(10):3437–3448. doi: 10.1016/j.bioactmat.2021.03.010

Fig. 4.

Fig. 4

In vitro analysis of osteogenesis on PT and GMPT scaffolds. (a) Schematic of the experiment. Cells were collected from each group after 7 days and 14 days of incubation. HUVECs were sorted into different groups and used in subsequent experiments. (b) Alizarin red staining images. (c) Quantification of Alizarin red staining through the absorbance value of osteogenesis of PT, 5% GMPT, 10% GMPT and 15% GMPT for 14 days. (d) ALP activity of PT, 5% GMPT, 10% GMPT and 15% GMPT on days 7 and 14. (e–h) The RT-PCR results of osteogenesis-associated gene expression of PT, 5% GMPT, 10% GMPT and 15% GMPT for 7 days and 14 days. The results demonstrated that the softer stiffness of GelMA provided a desirable surrounding environment for osteogenesis, among which 10% GMPT induced a higher level of osteogenic gene expression. Asterisks indicate significant differences, * represents P＜0.05, ** represents P＜0.01, *** represents P＜0.001 and **** represents P＜0.0001.