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Transfection scale depending on the size of a cell plate
| Cell culture dish | Platinum E
cells (miilion/plate) |
Media (ml) | Fugene 6 (μl) | Opti-MEM (μl) | DNA (μg) |
|---|---|---|---|---|---|
| 15 cm | 13.75 | 25 | 75 | 750 | 25 |
| 10 cm | 5.5 | 10 | 30 | 300 | 10 |
| 6 cm | 2.2 | 4 | 12 | 120 | 4 |
| 35 mm (6 well) | 1.1 | 2 | 6 | 60 | 2 |
| 12 well | 0.55 | 1 | 3 | 30 | 1 |
| 24 well | 0.275 | 0.5 | 1.5 | 15 | 0.5 |
