Fig. 5.
Representative fluorescence in situ hybridization (FISH) images of healthy control, CTG18.1 expansion-negative Fuchs endothelial corneal dystrophy (FECD) and CTG18.1 expansion-positive FECD corneal explant tissue. (CUG)n RNA foci detection was performed using Cy3-(CAG)7 probe using methods adapted from Zarouchlioti et al., (2018). White arrows highlight RNA foci within the CTG18.1 expansion-positive endothelium. y.o., years old. Scale bar for multiple nuclei, 20 μm (top panel). Single nuclei, 5 μm (lower panels).