Table 2.
Comparison of lysine posttranslational modifications at individual sites of skin type V collagen from P3H3 and LH1 null mice relative to their wild-type controls
| Lys (%) | p value | Hyl (%) | p value | GHL (%) | p value | GGHL (%) | p value | |||
|---|---|---|---|---|---|---|---|---|---|---|
| α1 K84 | P3H3 | WT (3) | 0.63 ± 0.04 | 0.06323 | 2.97 ± 0.09 | 0.05873 | 6.61 ± 0.37 | 0.23762 | 89.8 ± 0.36 | 0.05822 |
| Null (3) | 12.3 ± 7.94 | 3.95 ± 0.65 | 6.01 ± 0.56 | 77.6 ± 8.01 | ||||||
| LH1 | WT (4) | 1.33 ± 0.92 | 0.05917 | 2.28 ± 0.48 | 0.92243 | 6.56 ± 0.17 | 0.06623 | 89.9 ± 0.66 | 0.01655 | |
| Null (4) | 3.25 ± 1.38 | 2.30 ± 0.12 | 6.88 ± 0.21 | 87.6 ± 1.18 | ||||||
| α2 K87 | P3H3 | WT (3) | 0 | 0.02794 | 16.2 ± 1.95 | 0.17434 | 4.55 ± 0.22 | 0.26084 | 79.3 ± 1.88 | 0.0248 |
| Null (3) | 8.07 ± 4.14 | 18.6 ± 1.59 | 4.55 ± 0.17 | 69.0 ± 4.71 | ||||||
| LH1 | WT (4) | 7.87 ± 8.46 | 0.00938 | 7.13 ± 1.51 | 0.61402 | 5.07 ± 0.65 | 0.01839 | 79.9 ± 7.92 | 0.00827 | |
| Null (4) | 37.3 ± 1.00 | 6.68 ± 0.74 | 3.33 ± 0.67 | 52.7 ± 8.79 | ||||||
GGHL, glucosylgalactosyl hydroxylysine; GHL, galactosyl hydroxylysine; Hyl, unmodified hydroxylysine; Lys, unmodified lysine.
Values are given as means ± S.D. The number in the bracket next to the genotypes indicates biological replicates.
Lys + Hyl + GHL + GGHL = 100%. Values of modified and unmodified hydroxylysine and unmodified lysine were obtained using mass spectrometry.