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. 2020 Jul 22;12(2):e1622. doi: 10.1002/wrna.1622

TABLE 1.

Summary of current knowledge about metazoan TENTs

Subfamily Enzyme (synonyms) Activity RNA substrate Effect on target RNAs Reference
Vertebrates
TENT1 TENT1 (TUT1, U6 TUTase, Star‐PAP, PAPD2) Oligouridylation U6 snRNA Maturation Trippe et al. (2006)
Polyadenylation (controversial) Pre‐mRNA Stabilization Laishram and Anderson (2010), Mellman et al. (2008)
TENT2 TENT2 (GLD‐2, PAPD4, TUT‐2) Polyadenylation mRNA Activation of translationally dormant mRNA Barnard et al. (2004), Rouhana et al. (2005), Swanger et al. (2013), Udagawa et al. (2012), Yamagishi et al. (2016)
Monoadenylation miRNA Stabilization D'Ambrogio et al. (2012), Hojo et al. (2020), Katoh et al. (2009), Kim et al. (2016)
TENT3

TUT4 (TENT3A, PAPD3, ZCCHC11, KIAA0191)

TUT7 (TENT3B, PAPD6, ZCCHC6, KIAA1711)

Oligouridylation

mRNA

Histone mRNA

LINE‐1 mRNA

Pre‐miRNA

miRNA

Pre‐rRNA

Pol III‐ncRNAs

Viral RNA

TSS RNA

Degradation Chang et al. (2014, 2018), Faehnle et al. (2014), Gutiérrez‐Vázquez et al. (2017), Heo et al. (2009), Jones et al. (2012), Łabno et al. (2016), Lackey et al. (2016), Le Pen et al. (2018), Lim et al. (2014), Morgan et al. (2019, 2017), Mullen and Marzluff (2008), Pirouz et al. (2016, 2019), Piskounova et al. (2011), Thornton et al. (2014), Ustianenko et al. (2018, 2013, 2016), Warkocki, Krawczyk, et al. (2018)
Monouridylation Pre‐miRNA Processing Heo et al. (2012), Kim et al. (2015)
TENT4 TENT4A (PAPD7, POLS, TRF4‐1, TUT5, POLK) Polyadenylation (tethering assay) mRNA Not defined Ogami et al. (2013)
Mixed A/G tailing (with TENT4B)

mRNA

Viral RNA

Stabilization Hyrina et al. (2019), Kim et al. (2020), Lim et al. (2018), Mueller et al. (2019)
TENT4B (PAPD5, TRF4‐2, GLD‐4, TUT3) Polyadenylation

Pre‐rRNA

rRNA

Y RNA

hTR

Degradation Fasken et al. (2011), Lubas et al. (2011), Preker et al. (2011), Shcherbik et al. (2010), Sinturel et al. (2017), Sudo et al. (2016)
mRNA Stabilization Burns et al. (2011), Shin et al. (2017)
Oligoadenylation

snoRNA

scaRNA

miRNA

Maturation

Degradation

Berndt et al. (2012), Son et al. (2018)

Shukla et al. (2019)

Monoadenylation miRNA Degradation (Boele et al. (2014)
Mixed A/G tailing (with TENT4A)

mRNA

Viral RNA

Stabilization Hyrina et al. (2019), Kim et al. (2020), Lim et al. (2018), Mueller et al. (2019)
TENT5

TENT5A (FAM46A)

TENT5B (FAM46B)

TENT5C (FAM46C)

TENT5D (FAM46D)

Polyadenylation mRNA Stabilization (shown for TENT5C and TENT5B) Bilska et al. (2020), Herrero et al. (2020), Hu et al. (2020), Mroczek et al. (2012)
TENT6 MTPAP (TENT6, PAPD1, SPAX4) Polyadenylation Mt‐mRNA Stop codon generation, stabilization Anderson et al. (1981), Nagaike et al. (2005), Tomecki et al. (2004), Wilson et al. (2014)
Oligoadenylation Mt‐tRNA Maturation Fiedler et al. (2015), Pearce et al. (2017)
Caenorhabditis elegans
TENT1 USIP‐1 Oligouridylation U6 snRNA

Stabilization

Recycling

Rüegger et al. (2015)
TENT2 GLD‐2 Polyadenylation Oocyte mRNA

Activation

Stabilization

Kim et al. (2010), Nousch et al. (2014), Schmid et al. (2009), Suh et al. (2006)
TENT3 CDE‐1 (PUP‐1, CID‐1) Uridylation

siRNA

Viral RNA

Degradation Le Pen et al. (2018), van Wolfswinkel et al. (2009)
PUP‐2 Uridylation Pre‐miRNA Processing Lehrbach et al. (2009)
PUP‐3 Uridylation Preston et al. (2019)
MUT‐2 (RDE‐3) Tandem UG repeats addition

siRNA

Transposons

Trigger gene silencing Preston et al. (2019), Shukla et al. (2020)
TENT4 GLD‐4 Polyadenylation Oocyte mRNA Not defined Nousch et al. (2014)
TENT5 PQN‐44 (TENT5) Putative ncPAP
Drosophila melanogaster
TENT2 Gld2 Polyadenylation mRNA Activation Jae et al. (2008), Sartain et al. (2011)
Wispy Polyadenylation mRNA

Activation

Stabilization

Benoit et al. (2008), Coll et al. (2018), Cui et al. (2008, 2013), Dufourt et al. (2017), Eichhorn et al. (2016), Lim et al. (2016)
Oligoadenylation

miRNA

sisRNAs

Degradation

Stabilization

Lee et al. (2014), Jia Ng et al. (2018)
TENT3 Tailor Oligouridylation

Mirtrons

5S rRNA

tRNA

snRNA

snoRNA

RNase MRP

Degradation Bortolamiol‐Becet et al. (2015), Reimão‐Pinto et al. (2015), Reimão‐Pinto et al. (2016)
TENT4 Trf4‐1 Polyadenylation

snRNA

mRNA

Degradation Harnisch et al. (2016), Nakamura et al. (2008)
Trf4‐2 Inactive Nakamura et al. (2008)
TENT5 CG46385 Putative ncPAP