TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5’−3’) |
|---|---|
| pBT−0106‐EcoRI‐F | GAATTCTATGAATATTTATCAAAAAAACTTGCAAGCTCT |
| pBT−0106‐BglII‐R | AGATCTTTACCATTCTTTCAAAGCCATTTTGATC |
| pTRG‐FlaA‐BamHI‐F | GGATCCATGGCTTTTCAGGTCAATACAAATATCAAT |
| pTRG‐FlaA‐XhoI‐R | CTCGAGCTAAGTTAAAAGCCTTAAGATATTTTGTTGAACG |
| 0106‐Ser350toAla‐F | GGCATAGGCATGGCCGCAGCGAACATG |
| 0106‐Ser350toAla‐R | CATGTTCGCTGCGGCCATGCCTATGCC |
| 0106‐Phe376toAla‐F | GGGCAAGATTTGAGCGCTTCACAAAGCGGTAACA |
| 0106‐Phe376toAla‐R | TGTTACCGCTTTGTGAAGCGCTCAAATCTTGCCC |
| 0106‐Glu415toAla‐F | GGGGGTAATGGGAAAGTAGCAACCACT TTAGTGTGGAAA |
| 0106‐Glu415toAla‐R | TTTCCACACTAAAGTGGTTGCTACTTTCCCATTACCCCC |
| jhp0106‐Mut−1 | CCGGAATTCCGCTTCTTCAACTTGCGTTT |
| jhp0106‐Mut−2 | CCGGAATTCAAATCAACCAGCATTTCACG |
| gyrA‐RealT−1 | GACACCGCAGTTTATGATGC |
| gyrA‐RealT−1 | TTCTGGCTTCAGTGTAACGC |
| jhp0548(flaA)‐qPCR−1 | AATGGCGGTCAGGATTTAAC |
| jhp0548(flaA)‐qPCR−2 | TGTGAGTCAGAAGCCGAAAC |