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. 2021 Mar 18;21(5):395. doi: 10.3892/ol.2021.12656

Figure 6.

Figure 6.

Effects of albendazole treatment on oxidative stress-related and Wnt/β-catenin signaling genes in PC3 and DU145 cells. Following treatment with albendazole, gene expression in PC3 and DU145 cells was analyzed by reverse transcription-quantitative PCR and western blotting. (A) mRNA expression levels of the antioxidant enzymes CAT, GPX1, GPX3, CISD and HIF1A were downregulated by albendazole treatment (n=6). (B) The mRNA expression levels of CTNNB1, TWIST1, TCF4 and BCL2 were also downregulated by albendazole treatment (n=6). (C) Albendazole decreased TCF4 and BCL2 protein expression in PC3 cells. *P<0.05, **P<0.01 and ***P<0.001 vs. control. Results are presented as the mean ± SEM. CAT, catalase; GPX, glutathione peroxidase; CISD2, CDGSH iron sulfur domain 2; HIF1A, hypoxia-inducible factor 1α; CTNNB1, catenin β1; TWIST1, Twist family BHLH transcription factor 1; TCF4, transcription factor 4; ABZ, albendazole.