Figure 4.

The protective effect of edaravone on wound healing in a DM mouse model. (a) After wounds were created on the backs of mice with streptozotocin-induced diabetes, the percentage of the necrotic areas of the control, fibrin, and EDV with fibrin groups was compared one, three, and five days postoperatively. (b) After wound creation in diabetic mice, neutrophils/area (mm²) of the control, fibrin, and EDV with fibrin groups were compared five days postoperatively. (c) On postoperative days 1, 3, and 5, the H&E-stained cross-sections of the wounds in the control, 2 mg/mL fibrin, and 500 μM EDV with 2 mg/mL fibrin group were compared. (d) To determine the effect of EDV on the vascularity of the wound healing, CD31 staining was used to compare the control, 2 mg/mL fibrin, and 500 μM EDV with 2 mg/mL fibrin groups on days 1, 3, and 5 postoperatively. (e) The ratio of p-STAT3, STAT3, NOX3, p- NF-κB, NF-κB, and bcl-2 was calculated by Western blot analysis in the control, 2 mg/mL fibrin, 500 μM EDV with 2 mg/mL fibrin group on one, three, and five days after wound creation. (A color version of this figure is available in the online journal.)