Figure 6.

G protein-coupled estrogen receptor 1 (GPER1) activation improves proximal tubule brush-border integrity in high salt-fed female Dahl salt-sensitive (SS) rats. Representative images of proximal tubule brush-border integrity in female Dahl SS rats systemically treated with vehicle (Veh) (A) or G1 (400 μg/kg/day; B) and fed a high-salt diet for 2 wk. The areas indicated by squares within A and B are shown at higher magnification in C and D. Quantification of the brush-border integrity is graphed in E. Data are presented as means ± SE (n = 6 rats in each group). Statistical comparisons were performed by unpaired t test. Scale bars = 50 μm.