Figure 7.

Myoblast proliferation in tibialis anterior (TA) and flexor digitorum superficialis (FDS) muscles. The ratio of PAX7⁺ nuclei to total nuclei (A), PAX7⁺ nuclei per myofiber (B), Ki-67⁺ nuclei to total nuclei (C), and PAX7⁺ myoblasts that were also Ki-67⁺ to PAX7⁺ nuclei (D) were quantified in TA and FDS muscles for SAL (white bar, n = 5) and LR3 IGF-1 (gray bar, n = 7). Individual animal data and means ± SE are shown. Open circles (○) indicate a male fetus, and closed circles (●) indicate a female fetus. Significant effects from two-way ANOVA (LR3 IGF-1, muscle type) are indicated; Bonferroni post hoc test was performed. IGF-1, insulin-like growth factor-1; SAL, saline.