Figure 4.
Saxa suppresses ANG II-mediated activation of kidney immune cells. Surface expression of CD26 was measured by flow cytometry (A and B). ANG II-induced increases of CD26Bright CD8+ T lymphocytes (A) and CD26+ profibrotic macrophages (CD11b+F4/80−Ly6C−; B) in the kidney were attenuated by Saxa. Representative histogram of the macrophage data is shown on the right. C: dot plot representation of ANG II-induced increase of neutrophils in the kidney that was attenuated by Saxa. n = 6 mice per group. Statistical significance was determined using two-way ANOVA followed by Fisher’s LSD post hoc analysis. *P < 0.05 vs. all other groups, ‡P < 0.05 vs. ANG II, §P = 0.08 vs. Con. ANG II, angiotensin II; Con, control; Saxa, saxagliptin.