Table 2.
Kidney lymphoid immune cell populations by group
| Cell Type | Cell Gates | Control | Control + Saxagliptin | ANG II | ANG II + Saxagliptin |
|---|---|---|---|---|---|
| Leukocytes | CD45+ | 1.90 ± 0.21 | 2.17 ± 0.33 | 3.60 ± 0.49* | 3.36 ± 0.62* |
| CD4+ T cells | CD4+ | 1.00 ± 0.00 | 0.99 ± 0.08 | 0.81 ± 0.06* | 0.81 ± 0.06* |
| CD4+ CD26Lo | 50.80 ± 1.84 | 50.14 ± 1.69 | 54.62 ± 2.73 | 48.82 ± 2.52 | |
| CD4+ Memory T cells | CD4+ CD62L− CD44Hi | 1.00 ± 0.00 | 0.86 ± 0.05 | 1.06 ± 0.08 | 1.01 ± 0.10 |
| CD4+ CD62L− CD44Hi CD127+ | 1.00 ± 0.00 | 1.40 ± 0.29 | 1.65 ± 0.19* | 1.62 ± 0.15* | |
| CD4+ CD62L− CD44Hi CD127− | 1.00 ± 0.00 | 0.92 ± 0.05 | 0.93 ± 0.02* | 0.89 ± 0.02* | |
| CD4+ CD62L+ CD44+ | 1.00 ± 0.00 | 1.20 ± 0.23 | 1.27 ± 0.36 | 1.91 ± 0.41* | |
| CD8+ T cells | CD8+ | 1.00 ± 0.00 | 0.84 ± 0.05 | 0.98 ± 0.16 | 0.82 ± 0.12 |
| CD8+ CD26Hi | Median (Comp-PE-A) | 1.00 ± 0.00 | 1.06 ± 0.05 | 1.91 ± 0.45* | 0.95 ± 0.06‡ | |
| CD8+ Memory T cells | CD8+ CD62L− CD44Hi | 1.00 ± 0.00 | 0.84 ± 0.09 | 1.00 ± 0.18 | 0.73 ± 0.09 |
| CD8+ CD62L− CD44+ CD127+ | 1.00 ± 0.00 | 1.23 ± 0.18 | 1.14 ± 0.13 | 1.11 ± 0.08 | |
| CD8+ CD62L− CD44+ CD127− | 1.00 ± 0.00 | 0.90 ± 0.08 | 0.91 ± 0.05 | 0.94 ± 0.04 | |
| CD8+ CD62L+ CD44Hi CM | 1.00 ± 0.00 | 0.70 ± 0.15 | 1.30 ± 0.09 | 1.30 ± 0.32 | |
| CD8+ CD62L− CD44Hi CD26+ | 1.00 ± 0.00 | 0.98 ± 0.11 | 1.26 ± 0.15¥ | 1.02 ± 0.09 | |
| B cells | B&NK cells/CX3CR1− | 1.00 ± 0.00 | 1.04 ± 0.05 | 1.13 ± 0.08 | 1.21 ± 0.08* |
Values are means ± SE (absolute or relative to control); n = 4–6 mice per group. Statistical significance was determined using two-way ANOVA followed by Fisher’s LSD post hoc analysis. *P < 0.05 vs. control; ‡P < 0.05 vs. ANG II; ¥P = 0.05–0.09 vs. control. ANG II, angiotensin II.