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A variety of granules, protein assemblies, and large or small protein complexes can be separated in SEC. The range of particles separable using the CL2B SEC matrix can resolve large condensates, such as nuclear pore complexes and protein granules that associate with RNA Pol II during transcription. In the middle to late fractions elute large to small complexes of ordinary size, including ribosomes, RNA Pol II, and monomers.
