Fig. 3.

Validation of cell-to-clone assignment with gene expression for subject S144. a, b, c, d Transcript expression of the cells reduced to two dimensions using UMAP, shown separately for the cells in multiplet BCR clusters (a, b) and for cells belonging to singleton BCR clusters (c,d). Each point corresponding to a cell is colored by its clone assigned by CACTUS (a, c) and by cardelino [21] (b, d). The advantage of CACTUS in terms of agreement with gene expression is more pronounced for cells in multiplet BCR clusters