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. 2021 Mar 24;13:45. doi: 10.1186/s13073-021-00842-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — BCR cluster assignment to tumor clones, for both subjects. S144 (a, b) and S12118 (c, d), using CACTUS (a, c) and cardelino [21] (b, d). Heatmaps with shades of green indicate the proportion of cells in multiplet cluster (y axis) assigned to clones (x axis). Each number in a green entry indicates the non-zero number of cells of the corresponding BCR clusters assigned to the corresponding clone. Only BCR clusters of at least two cells are featured. As expected, for both subjects, CACTUS assigns entire BCR clusters to single clones (a, c). For cardelino, the proportions of BCR clusters are more distributed across the clones (b, d)