Fig. 4.

HDAC and Akt inhibitors exhibit cytotoxicity in several MM cell lines. a-j MTT proliferation assay. Each cell line was treated with DMSO, 2 μM ACY-1215 (ACY), 4 μM afuresertib (Afu), or a combination of ACY-1215 and afuresertib (Comb) for 72 h. The average was calculated for each experiment performed in quintuplicate (n = 5). (*p < 0.05, **p < 0.01, “ns” indicates no significant difference). k H929 were treated with 2 μM ACY-1215, 4 μM afuresertib with/without 1 μM CHIR 99021 (GSK-3 inhibitor) for 48 h. Experiments were performed in quintuplicate (n = 5). (**p < 0.01, ***p < 0.001)