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. 2021 Mar 23;21:88. doi: 10.1186/s12866-021-02148-8

Fig. 5.

Fig. 5

Recombinant proteins could be expressed in DualX-B15(DE3). a SDS-PAGE analysis of GST expressed in HT115(DE3), BL21(DE3) and DualX-B15(DE3) strains. b Western blot of GST expressed in DualX-B15(DE3). Lane M: prestained protein marker; Lane N: cells without the protein-expression plasmid; Lane U: uninduced cells with the protein-expression plasmid; Lane I: induced cells; Lane INS: insoluble fraction; Lane SOL: soluble fraction. The molecular weight of GST is 26 kDa as indicated by a red asterisk