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. 2021 Mar 23;21:88. doi: 10.1186/s12866-021-02148-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — dsRNA-VP28 and MrNV-CP could be co-expressed in DualX-B15(DE3). a The RNase digestion assays: untreated (U), RNase A (A) and RNase III (III)-treated samples. Lane M (Marker) is a 2-log DNA ladder. The molecular weight of dsRNA-VP28 is indicated by an arrow. b SDS-PAGE analysis of protein expression in the three different strains of E. coli. A red dot is shown to the right of the expected band of MrNV-CP. c Western blot was performed with anti-MrNV capsid protein monoclonal antibody. Lane M: a pre-stained protein marker; Lane N: cells without the protein-expression plasmid; Lane I: induced cells; Lane P: a positive control (purified MrNV capsid proteins); Lane INS: insoluble fraction; Lane SOL: soluble fraction. The molecular weight of MrNV capsid protein is 40 kDa as indicated by a red asterisk.