Fig. 5.

Mitochondrial transformation after 10 μM CCCP administration in MEFs and HeLa cells (Figure adapted from Figs. in ref. no. 4 / CC BY 4.0). Time-lapse images obtained by confocal microscopy (a). The number shown in figures denotes the number of seconds after administration. TEM images of mitochondria in HeLa cells 10 min after treatment with CCCP (b). Live 3D CLEM observation of MEFs after 10 min of CCCP treatment (c). Confocal microscopy image of the transformed mitochondria after time-lapse imaging and fixation (c-1). Virtual cross-section (c-2) and volume-rendered view (c-3) of the area corresponding to c-1. Virtual cross-sections of different levels of mitochondria, shown by red arrows in c-1 to c-3 (c-4). While section 466 shows a ring shape, section 473 shows that the lumen of the ring is connected to the outside, indicating that this mitochondrion is not a true ring shape in 3D. The transparent volume-rendered image demonstrated that this mitochondrion was not a ring, but rather a vase shape (c-5). Transparent volume-rendered images show that vase-shaped mitochondrion have an endoplasmic reticulum in the lumen of vase connected to the external cytoplasm (d). bar = 1 μm.