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The average charge state for ubiquitin (10 μM in acetic acid, pH 3.0) was determined from the spectra shown in the upper left by taking the weighted average of charge state as function of solution temperature. The midpoint at Tm = 71°C is in excellent agreement with 71 ± 2°C reported by Wintrode et al. (1994). Reproduced from El-Baba et al. (2017).
