FIGURE 18.
(A) Solidworks schematic of first generation FT-IM-PF-DT coupled to a Thermo Scientific Exactive Plus orbitrap MS with EMR that was used for the following section of experimentation Briefly, ion is generated via static-spray nano-ESI into a heated capillary at ~100°C. Ions are then transmitted into an RF ion funnel at (250 Vpp). The ion beam is modulated at both the Gate 1 and Gate 2 by a linear frequency chirp of 5–5,005 Hz over 8 min to overcome the duty-cycle mismatch of IM separation and MS analysis. (B) Resolved peaks for T4 and endogenous zinc binding to TTR and corresponding ATDs obtained from instrument shown in panel A. Figure is adapted from Poltash et al. (2018). ATD, arrival time distributions; DT, drift-tube; EMR, extended mass range; ESI, electrospray ionization; FT, Fourier-transform; IM, ion-mobility; PF, periodic-focusing; TTR, transthyretin.