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. 2021 Mar 22;20:15330338211004916. doi: 10.1177/15330338211004916

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Validation of TMEM47 expression on mRNA and protein level. (a) The relative expression of TMEM47 in TAMR/MCF-7 was validated by qRT-PCR and RNA Seq. (b) qRT-PCR was performed to validate TMEM47 expression in the 6 cell lines. The expression level of each gene was normalized to the level in MCF-7 cells. (****P < 0.0001) Results are representative of 3 independent experiments. (c) From 1 to 6 was TMEM47 expression in MCF-7, TAMR/MCF-7, Leti-control/MCF-7, TMEM47-OE/MCF-7, TAMR/MCF-7 transfected with NC and TAMR/MCF-7 transfected with siRNA. TMEM47 could not be detected in MCF-7 and Leti-control/MCF-7.