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. 2021 Mar 22;20:15330338211004916. doi: 10.1177/15330338211004916

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Construction of human TMEM47 overexpression in MCF-7 cell line (a) the map of pLOC vector with the restriction site NheI and Bam HI (b) The insert fragment was 550 bp after plasmid digestion. (c) Packaging efficiency in 293 T cells of lentivirus in control group and leti-control/MCF-7 cells after Blasticidin screening. Leti-control group was the vector without any fragment insert packaged in 293 T cells. Green fluorescence and red fluorescence could be detected in the control group. Blasticidin was used to screen for stable expression MCF-7 cells. (d) Packaging efficiency in 293 T cells of lentivirus in TMEM47 group and TMEM47-OE/MCF-7 cells after Blasticidin screening. TMEM47 was inserted into the pLOC vector; TMEM47 lentivirus plasmid and packaging plasmid were co-transferred to 293 T cells; TMEM47-OE/MCF-7 cells were obtained after Blasticidin screening. Green fluorescence could be detected.