Skip to main content
. 2021 Mar 5;13(3):197–209. doi: 10.1093/jmcb/mjab014

Figure 2.

Figure 2

Differentiation, activation, proliferation, and function of CD8+ T cells in peripheral blood from the HD and CR cohorts. (A) Gating strategy and the frequencies of Teff (CD45RO−CD27−), Tem (CD45RO+CD27−), Tcm (CD45RO+CD27+), and Tna (CD45RO−CD27+) subsets among CD8+ T cells in PBMCs. (B and C) Frequencies of HLA-DR+, PD-1+, and Ki-67+ CD8+ T cells. To analyze the function of CD8+ T cells, PBMCs were stimulated with PMA/ionomycin for 4.5 h in the presence of BFA and monensin. (D–H) The production of IL-2 (D, left panel), GZMB (D, right panel), IFN-γ (E, left panel), IFN-γ/IL-2, IFN-γ/GZMB (F), IL-4 (G), and IL-17A (H) by CD8+ T cells were analyzed by intracellular staining. T-bet+ cells among CD8+ T cells without stimulation (E, middle panel) and the correlation of T-bet+ with IFN-γ+ (E, right panel) are shown. Data were obtained from 55 healthy donors and 55 COVID-19 recovered individuals, except that data on IL-2 (D) and IFN-γ/IL-2 (F) were obtained from 55 healthy donors and 36 COVID-19 recovered individuals. ns, nonsignificant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.