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. 2021 Mar 19;14:2065–2074. doi: 10.2147/OTT.S268056

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© 2021 Lei et al.

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Effect of treatment with HP on apoptosis of Dami cells. (A) Flow cytometric analysis of apoptotic Dami cells after treated with HP at 0h, 3h, 6h, 9h; Statistical analysis was obtained by calculating the percentage of apoptotic population from three independent experiments on Dami cells, respectively; (B) TEM analysis of apoptotic Dami cells after treated with HP at 0h, 3h, 6h, 9h; Scale bars: 1 um; (C) TUNEL staining of apoptotic Dami cells after treated with HP at 0h, 3h, 6h, 9h; Scale bars: 100 um; (D) relative mRNA expression levels of apoptosis-related genes (Bax and Bcl-2) were measured in Dami cells by qPCR analysis; (E) protein levels of Bax and Bcl-2 were detected in Dami cells by Western blot analysis; All data are expressed as mean ± SEM (n≥3). *p<0.05, **p<0.01, ***p<0.001 versus vehicle. Analysis of Variance (ANOVA) and Student’s t-test (two-tailed).