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. 2021 Mar 19;14:2065–2074. doi: 10.2147/OTT.S268056

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© 2021 Lei et al.

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Treatment with HP promotes apoptosis of Dami cells through the NF-κB/IL-17 pathway. (A) Relative mRNA expression levels of apoptosis-related genes (P65 and IL-17) were measured in Dami cells by qPCR analysis; (B) protein levels of IL-17 were detected in culture supernatation of Dami cells by ELISA analysis; (C) protein levels of total P65, p-P65, p-IKBα, IL-17 and GAPDH were detected in Dami cells by Western blot analysis; (D) flow cytometric analysis of apoptotic Dami cells. The difference of apoptosis rates among four groups was analyzed; (E) TEM analysis of apoptotic Dami cells; Scale bars: 1 um; (F) TUNEL staining of apoptotic Dami cells; Scale bars: 100 um; (G) protein levels of IL-17 were detected in culture supernatation of Dami cells by ELISA analysis; (H) relative mRNA expression levels of apoptosis-related genes (Bax and Bcl-2) were measured in Dami cells by qPCR analysis; (I) protein levels of total P65, p-P65, p-IKBα, IL-17 and GAPDH were detected in Dami cells by Western blot analysis; All data are expressed as mean ± SEM (n≥3). *p<0.05, **p<0.01, ***p<0.001 versus vehicle. Analysis of Variance (ANOVA) and Student’s t-test (two-tailed).