Table 3.
Chemical inhibitors used in this study
| Chemical inhibitor | Effect | Concentration |
|---|---|---|
| NH4Cl | Inhibits pH-dependent activation of the fusion protein | 10 mM/20 mM |
| Bafilomycin A1 | Vacuolar-type ATPase inhibitor | 10 nM/20 nM |
| Chlorpromazine | Inhibits clathrin-dependent endocytosis | 5 μM/10 μM |
| Pitstop-2 | Cell-permeable clathrin inhibitor | 5 μM/10 μM |
| Nystatin | Disrupts caveolae | 12.5 μg/25 μg |
| MβCD | Extracts cholesterol from lipid membranes | 2.5 mM/5 mM |
| Dynasore | Inhibits GTPase activity | 20 μM/40 μM |
| Mitmab | Blocks the lipid binding | 5 μM/10 μM |
| Cytochalasin D | Disrupts actin polymerization | 5 μM/10 μM |
| Jasplakinolide | Stabilizes actin microfilaments | 5 μM/10 μM |
| EIPA | NA+/H+ exchanger inhibitor | 10 μM/20 μM |
| IPA-3 | the Pak-1 inhibitor | 7.5 μM/15 μM |
| Wortmannin | the PI3K inhibitor | 5 μM/10 μM |