Table 1.

Primer sequences used for qPCR analysis.

Primer	Sequence	Tm
Eubacteria FP	5′-ACTCCTACGGGAGGCAGCAGT-3′	68.4 °C
Eubacteria RP	5′-ATTACCGCGGCTGCTGGC-3′	70.7 °C
Enterobacteriaceae FP	5′-GTGCCAGCMGCCGCGGTAA-3′	76.3 °C
Enterobacteriaceae RP	5′-GCCTCAAGGGCACAACCTCCAAG-3′	73.6 °C
Bacteroidetes FP	5′-GGTTCTGAGAGGAGGTCCC-3′	62.7 °C
Bacteroidetes RP	5′-GCTGCCTCCCGTAGGAGT-3′	64.4 °C
Firmicutes FP	5′-GGAGYATGTGGTTTAATTCGAAGCA-3′	63.9 °C
Firmicutes RP	5′-AGCTGACGACAACCATGCAC-3′	66.5 °C
Actinobacteria FP	5′-CGCGGCCTATCAGCTTGTTG-3′	69.9 °C
Actinobacteria RP	5′-CCGTACTCCCCAGGCGGGG-3′	74.9 °C

FP, forward primer; RP, reverse primer; Tm, melting temperature.