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. 2021 Mar 19;13:2669–2680. doi: 10.2147/CMAR.S275352

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© 2021 Guo et al.

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PROX1-AS1 knockdown suppresses the proliferation and promotes apoptosis of GC cells. (A) Transfection efficiency was verified by RT-qPCR analysis. (B) Cell viability was examined by CCK-8 assay. (C) The functional role of PROX1-AS1 on colony formation was analyzed by colony formation assay (magnification, 40×). (D) Cell cycle was assessed by flow cytometry assay. (E) The expression of cell cycle regulatory proteins was analyzed using Western blot. (F) Cell apoptosis was assessed by flow cytometry assay. (G) The expression of cell apoptosis regulatory proteins was analyzed using Western blot. *P < 0.05, **P < 0.01, ***P < 0.001 vs sh-NC group.