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. 2021 Mar 19;13:2669–2680. doi: 10.2147/CMAR.S275352

Figure 6.

Figure 6

PD-L1 is target gene of miR-877-5p. (A) The predicted binding site of miR-877-5p in the 3ʹ-UTR of PD-L1. (B) The relative luciferase activity was detected. (C, D) Relative expression of PD-L1 was examined by RT-qPCR and Western blot assay. **P < 0.01 vs NC mimic. (E, F) The mRNA expression of PD-L1 in GC tissues and cell lines were analyzed by RT-qPCR. (G) Pearson’s correlation analysis between miR-877-5p and PD-L1 expression. *P < 0.05, **P < 0.01, ***P < 0.001 vs NC mimics, Non-tumor, or GES-1 group.