KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-HA-Tag (C29F4) | Cell Signaling Technologies | Cat#3724; RRID:AB_1549585 |
| Rabbit anti-cGAS (Mouse specific) (D3O8O) | Cell Signaling Technologies | Cat#31659; RRID:AB_2799008 |
| Rabbit anti-STING (D2P2F) | Cell Signaling Technologies | Cat#13647; RRID:AB_2732796 |
| Rabbit anti-MDA-5 (D74E4) | Cell Signaling Technologies | Cat#5321; RRID:AB_10694490 |
| Rabbit anti-Phospho-IRF-3 (Ser396) (4D4G) | Cell Signaling Technologies | Cat#4947; RRID:AB_823547 |
| Rabbit anti-IRF-3 (D83B9) | Cell Signaling Technologies | Cat#4302; RRID:AB_1904036) |
| Rabbit anti-STAT1 (D1K9Y) | Cell Signaling Technologies | Cat#14994; RRID:AB_2737027 |
| Rabbit anti-TBK1 (D1B4) | Cell Signaling Technologies | Cat#3504; RRID:AB_2255663 |
| rabbit anti-Phospho-TBK1 (S172) (D52C2) | Cell Signaling Technologies | Cat#5483; RRID:AB_10693472 |
| Mouse anti-Viperin | Millipore | Cat#MABF106 RRID:AB_11203644 |
| Goat anti-Rabbit 800CW | LI-COR | Cat#926-32211; RRID:AB_621843 |
| Goat anti-Mouse 800CW | LI-COR | Cat#827-08364; RRID:AB_10793856 |
| Critical commercial assays | ||
| REVERT Total Protein Stain Kit | LI-COR | Cat#926-11010 |
| ATPlite Luminescence Assay System | Perkin Elmer | Cat#6016941 |
| MycoAlert Mycoplasma Detection Kit | Lonza Bioscience | Cat#LT07-218 |
| Deposited data | ||
| Raw and analyzed RNA sequencing data | This paper | GEO: GSE163313 |
| Full immunoblots for the cropped immunoblots in Figures 1, 3, 4, and S1. | This paper | Mendeley Data: https://dx.doi.org/10.17632/5348dspthz.1 |
| Experimental models: cell lines | ||
| Rev3l HET 1 MEFs: Rev3L(+/Δ) mT/mG (+/−) MEFs from mouse embryo 3, TAg immortalized clone 6 (or 3(+)6) | Lange et al., 2012, 2016 | N/A |
| Rev3l HET 2 MEFs: same as above except clone 10 (or 3(+)10) | Lange et al., 2012, 2016 | N/A |
| Rev3l KO 1 MEFs: Rev3L(−/Δ) mT/mG (+/−) MEFs from mouse embryo 4, TAg immortalized clone 5 (or 4(-)5) | Lange et al., 2012, 2016 | N/A |
| Rev3l KO 2 MEFs: same as above except clone 10 (or 3(+)10) | Lange et al., 2012, 2016 | N/A |
| Rev3l HET + EV #Clone: HET 1 + pCDH-EF1-Flag-HA-Empty, clones: 4, 8 and 11 | This study | N/A |
| Rev3l KO + EV #Clone: KO 1 + pCDH-EF1-Flag-HA-Empty, clones: 3, 4, and 15 | This study | N/A |
| Rev3l KO + TR4-2 #Clone: KO 1 + pCDH-EF1-Flag-HA-TR4-2, clones: 8, 18, and 21 | This study | N/A |
| Oligonucleotides | ||
| “siCTL”: Negative Control DsiRNA Duplex | Integrated DNA Technologies | Cat#51-01-14-03 |
| “sicGAS”: cGAS (MB21D1) DsiRNA Duplex | Integrated DNA Technologies | Design ID: mm.Ri.Mb21d1.13.1 |
| “siSTING”: STING (TMEM173) DsiRNA Duplex | Integrated DNA Technologies | Design ID: mmRi.Tmem173.13.2 |
| For primers used for qPCR in this study, see Table S2. | See Table S2 | N/A |
| Recombinant DNA | ||
| pLEXm-His8-MBP-TR4-2 | Lee et al., 2014 | N/A |
| pCDH-EF1-Flag-HA-TR4-2 | This study | N/A |
| pCDH-EF1-Flag-HA-Empty | Tomida et al., 2015 | N/A |
| Software and algorithms | ||
| TopHat (version 2.0.10) | Kim et al., 2013 | N/A |
| HTSeq package (version 0.6.0) | Anders et al., 2015 | N/A |
| R/Bioconductor package edgeR (version 3.8.6) | Robinson et al., 2010 | N/A |
| Prism (version 8) | GraphPad | N/A |