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. Author manuscript; available in PMC: 2021 Mar 24.
Published in final edited form as: Methods Mol Biol. 2018;1790:113–125. doi: 10.1007/978-1-4939-7860-1_9

Fig. 2.

Fig. 2

Short half-life reporter gene activity. (a) Box and line schematic showing the short half-life luciferase reporter construct under the control of five tandem repeats of HRE. (b) Schematic showing binding of the transcription factor to the HRE and activating luciferase gene transcription that can be visualized as bioluminescence when the supplied substrate is converted by luciferase. (c) Luciferase assay quantifying the activity of the enzyme in PC3-HRE-NLC-ODD-Luc cells in response to 48 h of true hypoxia (1% oxygen). Values represent mean ± SEM from three independent experiments. *** p < 0.0005. (d) Representative image showing bioluminescence in PC3-HRE-NLS-ODD-Luc cells in response to 48 h treatment with hypoxia mimetic CoCl2. Images were captured using IVIS Spectrum Xenogen system