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. Author manuscript; available in PMC: 2022 Mar 19.
Published in final edited form as: Vaccine. 2021 Feb 23;39(12):1780–1787. doi: 10.1016/j.vaccine.2021.01.071

Fig. 5. Mtb-specific production of multiple antimicrobial cytokines by pulmonary T cells generated following neonatal BCG prime/intranasal MVA boost vaccination.

Fig. 5.

Cells from lung-associated lymph nodes of mice immunized as described in Fig. 2 were analyzed for concomitant secretion of IFNγ, IL-2 and IL-17 by ICS as described in Materials and Methods. Ag85A-specific (A) and ESAT-6-specific (B) CD4+ T cell, and Ag85A-specific CD8+ T cell (C) cytokine secretion profiles for triple- (3+), dual- (2+) and single-cytokine (1+) secretion, and comparisons between vaccination groups, are shown. Data represent mean counts of Mtb peptide-responsive cells per million CD4+ T cells ± SEM from five mice per group and are representative of two independent experiments. Statistical significance of differences between vaccine groups was determined using one-way ANOVA with Tukey’s correction for multiple comparisons and are denoted as *** (P < 0.001), ** (P < 0.01), or * (P < 0.05).