Table 2. Genotype and allele frequencies for each replicate of the KR x ROCK experiment and their associated Hardy-Weinberg (HWE) and genetic drift p-values.
Gen. | Sample size | Observed genotype frequency (expected#) | Allele frequency | HWE (p-value) | Genetic drift (p-value) | |||
---|---|---|---|---|---|---|---|---|
SS | RS | RR | S | R | ||||
AF1 | 90 | 0.07 | 0.93 | 0.00 | 0.53 | 0.47 | -- | -- |
BF1 | 88 | 0.10 | 0.90 | 0.00 | 0.55 | 0.45 | -- | -- |
A1F3 | 89 | 0.37 (0.39) | 0.51 (0.47) | 0.12 (0.14) | 0.62 | 0.38 | 0.015* | 0.000 |
A1F5 | 89 | 0.31 (0.33) | 0.52 (0.49) | 0.17 (0.18) | 0.57 | 0.43 | 0.142 | 0.002 |
A1F7 | 85 | 0.45 (0.51) | 0.53 (0.41) | 0.02 (0.08) | 0.71 | 0.29 | 0.000* | 0.000 |
A1F9 | 178 | 0.60 (0.61) | 0.37 (0.34) | 0.04 (0.05) | 0.78 | 0.22 | 0.006* | 0.000 |
A2F3 | 89 | 0.29 (0.36) | 0.61 (0.48) | 0.10 (0.16) | 0.60 | 0.40 | 0.005* | 0.000 |
A2F5 | 88 | 0.33 (0.34) | 0.51 (0.49) | 0.16 (0.17) | 0.59 | 0.42 | 0.564 | 0.273^ |
A2F7 | 87 | 0.47 (0.50) | 0.45 (0.42) | 0.08 (0.09) | 0.70 | 0.30 | 0.003* | 0.000 |
A2F9 | 180 | 0.30 (0.34) | 0.57 (0.49) | 0.13 (0.17) | 0.58 | 0.42 | 0.000* | 0.000 |
B1F3 | 88 | 0.26 (0.28) | 0.52 (0.49) | 0.22 (0.23) | 0.52 | 0.48 | 0.378 | 0.047 |
B1F5 | 89 | 0.50 (0.39) | 0.35 (0.47) | 0.20 (0.14) | 0.62 | 0.38 | 0.000* | 0.000 |
B1F7 | 89 | 0.43 (0.44) | 0.47 (0.45) | 0.10 (0.11) | 0.66 | 0.34 | 0.244 | 0.011 |
B1F9 | 90 | 0.50 (0.48) | 0.40 (0.43) | 0.11 (0.10) | 0.69 | 0.31 | 0.330 | 0.059^ |
B2F3 | 88 | 0.30 (0.27) | 0.44 (0.50) | 0.26 (0.23) | 0.52 | 0.48 | 0.152 | 0.023 |
B2F5 | 89 | 0.41 (0.44) | 0.50 (0.44) | 0.09 (0.12) | 0.66 | 0.34 | 0.000* | 0.000 |
B2F7 | 93 | 0.47 (0.45) | 0.40 (0.44) | 0.13 (0.11) | 0.67 | 0.33 | 0.391 | 0.189^ |
B2F9 | 90 | 0.42 (0.45) | 0.50 (0.44) | 0.08 (0.11) | 0.67 | 0.33 | 0.197 | 0.480^ |
Global χ2 (df = 32) = | 143.28 | 198.07 | ||||||
Global p-value = | 0.000* | 0.000 |
# Expected genotype frequency values estimated from same generation
* Values out of HWE
^ Unable to reject null hypothesis of allele frequency changes due to genetic drift