Table 3. Genotype and allele frequencies for each replicate of the CKR x ROCK experiment and their associated Hardy-Weinberg (HWE) and genetic drift p-values.
Gen. | Sample size | Genotype frequency observed (expected#) | Allele frequency | HWE (p-value) | Genetic drift (p-value) | |||
---|---|---|---|---|---|---|---|---|
SS | RS | RR | S | R | ||||
AF1 | 90 | 0.00 | 1.00 | 0.00 | 0.50 | 0.50 | -- | -- |
BF1 | 90 | 0.00 | 1.00 | 0.00 | 0.50 | 0.50 | -- | -- |
A1F3 | 90 | 0.33 (0.33) | 0.48 (0.49) | 0.19 (0.19) | 0.57 | 0.43 | 0.047* | 0.000 |
A1F5 | 90 | 0.33 (0.36) | 0.53 (0.48) | 0.13 (0.16) | 0.60 | 0.40 | 0.184 | 0.057^ |
A1F7 | 90 | 0.40 (0.46) | 0.51 (0.44) | 0.07 (0.11) | 0.68 | 0.32 | 0.014* | 0.000 |
A1F9 | 90 | 0.41 (0.37) | 0.38 (0.47) | 0.21 (0.17) | 0.60 | 0.40 | 0.001* | 0.000 |
A2F3 | 90 | 0.19 (0.22) | 0.57 (0.50) | 0.24 (0.28) | 0.47 | 0.53 | 0.142 | 0.059^ |
A2F5 | 90 | 0.29 (0.32) | 0.56 (0.49) | 0.15 (0.19) | 0.57 | 0.43 | 0.116 | 0.000 |
A2F7 | 90 | 0.39 (0.41) | 0.50 (0.46) | 0.11 (0.13) | 0.64 | 0.36 | 0.046* | 0.000 |
A2F9 | 89 | 0.47 (0.53) | 0.51 (0.40) | 0.02 (0.08) | 0.73 | 0.27 | 0.002* | 0.000 |
B1F3 | 90 | 0.29 (0.27) | 0.46 (0.50) | 0.26 (0.24) | 0.52 | 0.48 | 0.340 | 0.164^ |
B1F5 | 90 | 0.33 (0.36) | 0.53 (0.48) | 0.13 (0.160 | 0.60 | 0.40 | 0.018* | 0.000 |
B1F7 | 90 | 0.47 (0.49) | 0.47 (0.42) | 0.07 (0.09) | 0.70 | 0.30 | 0.020* | 0.000 |
B1F9 | 87 | 0.60 (0.60) | 0.36 (0.35) | 0.05 (0.05) | 0.78 | 0.22 | 0.029* | 0.000 |
B2F3 | 90 | 0.42 (0.42) | 0.46 (0.45) | 0.12 (0.12) | 0.65 | 0.35 | 0.000* | 0.000 |
B2F5 | 90 | 0.44 (0.42) | 0.41 (0.45) | 0.14 (0.12) | 0.65 | 0.35 | 0.378 | 0.506^ |
B2F7 | 90 | 0.54 (0.57) | 0.44 (0.36) | 0.03 (0.07) | 0.75 | 0.25 | 0.002* | 0.000 |
B2F9 | 90 | 0.68 (0.68) | 0.29 (0.29) | 0.03 (0.03) | 0.82 | 0.18 | 0.035* | 0.000 |
Global χ2 (df = 32) = | 125.46 | 237.43 | ||||||
Global p-value = | 0.000* | 0.000 |
# Expected genotype frequency values estimated from same generation
* Values out of HWE
^ Unable to reject null hypothesis of allele frequency changes due to genetic drift