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S. pombe strains bearing the indicated ppn1 truncation alleles in combination with rpb1-CTD phospho-site mutants were spot-tested for growth on YES agar at the indicated temperatures. The ppn1-(1–496) allele, which did not complement the cs growth phenotype seen in ppn1Δ, was lethal in the rpb1-Y1F, -S2A, and -T4A backgrounds.
