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S. pombe strains bearing the indicated ppn1 truncation alleles in combination with CPF subunit, Rhn1, or Pin1 mutants as specified were spot-tested for growth on YES agar at the indicated temperatures. The ppn1-(1–496) allele was synthetically lethal with ctf1Δ, rhn1Δ, ssu72-C13S, and pin1Δ.
