Fig 12. Effect of Dis2 and Swd22 binding-defective mutations on Ppn1 activity in vivo.

ppn1 mutants specified in the top row were crossed to the mutant strains specified in the left-most column. Synthetically lethal pairs of alleles are highlighted in red boxes. The yellow boxes indicate severe synthetic growth defects (very sick). A viable double mutant without a synthetic defect is indicated by a plain green box. A viable double mutant that displayed temperature-sensitive (ts) or cold-sensitive (cs) defects is annotated as such in its green box.