Fig 13. Sds21 can interact with Ppn1 via the Dis2 binding site.

(A) S. pombe wild-type, sds21Δ, and sds21Δ ssu72-C13S strains were spot-tested for growth on YES agar at the indicated temperatures. (B) S. pombe wild-type, dis2Δ, and sds21Δ cells were grown in liquid culture at 30°C and assayed for acid phosphatase activity. (C) Full-length Ppn1 (WT and the indicated mutants) fused to the Gal4 AD were tested for 2-hybrid interactions with Gal4 BD fusions to full-length Sds21. AD/BD pairs that scored positive in both reporter assays (LacZ expression and histidine prototrophy) are indicated as ++. Pairs that were negative in both reporter assays are scored as --.