Table 1. Genes and probes used in the Real time RT-PCR multiplex to detect SARS-CoV-2 using GAPDH as an internal control.
Target gene | Primer / Probe | Sequence 5’ → 3’ |
---|---|---|
RdRp | RdRp_SARSr-F | GTGARATGGTCATGTGTGGCGG |
RdRp_SARSr-P2 | FAM-CAGGTGGAACCTCATCAGGAGATGC-BBQ | |
RdRp_SARSr-R | CARATGTTAAASACACTATTAGCATA | |
GAPDH | GAPDH-F | GTGAAGGTCGGAGTCAACGG |
GAPDH-P | ROX-CGCCTGGTCAACAGGGTCGC-BBQ | |
GAPDH-R | TCAATGAAGGGGTCATTGATG |