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. 2021 Mar 24;16(3):e0248885. doi: 10.1371/journal.pone.0248885

Table 1. Genes and probes used in the Real time RT-PCR multiplex to detect SARS-CoV-2 using GAPDH as an internal control.

Target gene Primer / Probe Sequence 5’ → 3’
RdRp RdRp_SARSr-F GTGARATGGTCATGTGTGGCGG
RdRp_SARSr-P2 FAM-CAGGTGGAACCTCATCAGGAGATGC-BBQ
RdRp_SARSr-R CARATGTTAAASACACTATTAGCATA
GAPDH GAPDH-F GTGAAGGTCGGAGTCAACGG
GAPDH-P ROX-CGCCTGGTCAACAGGGTCGC-BBQ
GAPDH-R TCAATGAAGGGGTCATTGATG