Fig 4. Exposure of β-actin on the plasma membrane of neurons.

Immunofluorescence microscopy analysis showing fixed and non-permeabilized neurons and stained for nile red for the detection of plasma membrane lipids; neurons were co-stained with anti-β-actin antibody (A), anti-ALK antibody (B) and anti-⍺-tubulin 1B antibody (C). 3D-orthogonal views were used to assess whether the detection of β-actin antibody (A), anti-ALK antibody (B) and anti-⍺-tubulin 1B (C) was above the lipid layer of neuronal plasma membrane. Per each experimental group, n = 200 neurons (among two different biological replicates) were imaged.