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. 2021 Mar 24;17(3):e1009432. doi: 10.1371/journal.ppat.1009432

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© 2021 Tabusi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 9 — Immunofluorescence STED super-resolution microscopy analysis showing β-actin filaments (red) within neurons uninfected (A), infected with TIGR4ΔrrgA-srtDΔply (B) and TIGR4 (C). (D) Intensity profiles of the β-actin filaments selected within white dashed rectangles; fluorescent intensity profile measurement performed with the function of Image J Analyze/Plot Profile. the intensity profiles shown in D are representatives of the whole imaging experiment (for each experimental group, 25 neuronal cells were imaged). White arrows in C point towards gaps of β-actin filaments that correspond to the drop of fluorescent intensity measured in the intensity profile graph (black arrows, third graph from the left).